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KMID : 1101420170490030271
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Korean Journal of Clinical Laboratory Science
2017 Volume.49 No. 3 p.271 ~ p.278
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The Protective Effect of Lonicerae flos Extract on Cultured C6 Glioma Cells Damaged by Aluminum of Dementia Inducer
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Jung Jai-Yun
Jung In-Ju
Jekal Seung-Joo
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Abstract
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This study was performed to evaluate the cytotoxicity of aluminum chloride (AlCl3), and the protective effect of Lonicerae flos (LF) extract on AlCl3-induced cytotoxicity in the cultured C6 glioma cells. Here, the cell viability and antioxidative effects, such as DPPH-radical scavenging activity, superoxide anion scavenging activity, and lipid peroxidation (LP), were assessed. AlCl3 significantly decreased the cell viability in a dose-dependent manner; the XTT50 value was measured at 128.8 μM of AlCl3. The cytotoxicity of AlCl3 was determined as highly toxic the y Borenfreund and Puerner’s toxic criteria. The butylated hydroxytoluene (BHT) and antioxidant both significantly increased the cell viability, which was damaged by AlCl3-induced cytotoxicity in these cultures. In the protective effect of LF extract on AlCl3-induced cytotoxicity, the LF extract significantly increased the superoxide anion scavenging activity and inhibitory activity of LP, as well as the DPPH-radical scavenging activity. From these results, it is suggested that the oxidative stress may have been involved in the cytotoxicity of AlCl3, and LP extract effectively protected AlCl3-induced cytotoxicity through the antioxidative effects. Conclusively, the natural resources, like LP extract, may be a putative therapeutic agent for the treatment of dementia induced by allergen like aluminum correlated with the oxidative stress.
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KEYWORD
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Aluminum, Dementia, Cytotoxicity, Antioxidative effect
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